Structural transitions in ribonucleic acid during ribosome development.

Ribosome development and the methylation of ribosomal ribonucleic acid.

Immature ribonucleoprotein particles accumulate in amino acid-starved cells of a relaxed mutant of Escherichia coli. The ribonucleic acid (RNA) of these particles is nonmethylated in cells starved for methionine. However, in bacteria starved for arginine, lysine, or histidine, the RNA of these particles is one-half methylated. The relationship of submethylation to a structural alteration in the...

Studies on the Formation of Transfer Ribonucleic Acid-Ribosome Complexes I. THE EFFECT OF STREPTOMYCIN AND RIBOSOMAL DISSOCIATION ON “C-AiCIINOACYL TRANSFER RIBONUCLEIC ACID BINDING TO RIBOSOMES*

Streptomycin-sensitive 70s ribosomes which are dissociated into SOS and 30s particles by dialysis against 1 X lop4 M magnesium acetate, and then reassociated in 0.02 M magnesium acetate, differ from nondissociated 70s ribosomes in r4C-aminoacyl soluble ribonucleic acid (sRNA) binding. 1. Reassociated ribosomes bind about twice as much 14C!phenylalanyl-sRNA in the presence of polyuridylic acid a...

Structural determinants of an internal ribosome entry site that direct translational reading frame selection

The dicistrovirus intergenic internal ribosome entry site (IGR IRES) directly recruits the ribosome and initiates translation using a non-AUG codon. A subset of IGR IRESs initiates translation in either of two overlapping open reading frames (ORFs), resulting in expression of the 0 frame viral structural polyprotein and an overlapping +1 frame ORFx. A U-G base pair adjacent to the anticodon-lik...

Indirect evidence for the presence of Ribonucleic acid in vesicular stomatitis Virions

Improved methods for purification and assay of eukaryotic messenger ribonucleic acids and ribosomes. Quantitative analysis of their interaction in a fractionated reticulocyte cell-free system.

The polyadenylic acid-containing messenger ribonucleic acids of eukaryotic cells are rapidly isolated and deproteinized in a simple and gentle one-step procedure. The polyribosome fraction, dissolved in 0.5 M NaCl/0.5 percent sodium dodecyl sulfate, is passed through an oligo(dT)-cellulose column which is then washed with the solvent until proteins and contaminating ribonucleic acids are fully ...